Protein Phosphatase 1 beta (PPP1CB) Antibody (C-term)

SKU: orb1928505

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:10~50, WB: 1:1000
• Reactivity: Human
• Predicted Reactivity: Mouse, Other, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB06024
• Target: This Protein Phosphatase 1 beta (PPP1CB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human Protein Phosphatase 1 beta (PPP1CB).
• Molecular Weight: 37187 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Serine/threonine-protein phosphatase PP1-beta catalytic subunit, PP-1B, PPP1CD, PPP1CB

Western blot analysis of PPP1CB (arrow) using rabbit polyclonal PPP1CB Antibody (K301). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPP1CB gene.

Fluorescent image of U251 cell stained with PPP1CB Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PPP1CB primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).PPP1CB immunoreactivity is localized to Cytoplasm and Nucleus significantly.

Quick Database Links

Gene Symbol

This Protein Phosphatase 1 beta (PPP1CB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human Protein Phosphatase 1 beta (PPP1CB).

UniProt

P62140

RefSeq (Protein):NP_002700.1, NP_996759.1