Propranolol hydrochloride (b)

SKU: orb1225318

Description

Propranolol Hydrochloride is a widely used non-cardioselective beta-adrenergic antagonist.(In Vitro):Propranolol hydrochloride (10-7 M-10-3 M; 24 and 48 hours) increases the total ERK1/2 levels in a dose-dependent manner, and ERK1/2 activation is observed specifically at 10-5 M in HemSCs.Propranolol hydrochloride (10-9 M-10-3 M; 24 and 48 hours) significant decreases cell proliferation at 10-4 M propranolol after 24 hours and 10-9 M propranolol after 48 hours in HemSCs.Propranolol hydrochloride (50 μM-200 μM; 24 hours) increases Annexin V positivity and caspase-3 activation, rapidly induces HemSC apoptosis.(In Vivo):Propranolol hydrochloride (orally administration; 40 mg/kg; daily) significantly reduces the vessel diameter relative to the vehicle-treated implants, and increases the number of cells that expressed phosphorylated ERK1/2 within the IH Matrigel implant.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 318-98-9
• MW: 295.81
• Purity: >98% (HPLC)
• Formula: C₁₆H₂₂ClNO₂
• SMILES: Cl.CC(C)NCC(O)COC1=CC=CC2=CC=CC=C12
• Target: Adrenergic Receptor
• Solubility: Water: 6 mg/mL (20.28 mM); DMSO: 59 mg/mL (199.45 mM)

Bioactivity

• In Vivo:
  Propranolol hydrochloride (orally administration; 40 mg/kg; daily) significantly reduces the vessel diameter relative to the vehicle-treated implants, and increases the number of cells that expressed phosphorylated ERK1/2 within the IH Matrigel implant. Animal model: A xenograft mouse model of IH (infantile hemangiomas) with HemSC cells. Dosage: 40 mg/kg. Administration: Orally administration; 40 mg/kg; daily. Result: Improved vessel development in the IH mouse model that correlated with MAPK pathway activation.
• In Vitro:
  Propranolol hydrochloride (10-7 M-10-3 M; 24 and 48 hours) increases the total ERK1/2 levels in a dose-dependent manner, and ERK1/2 activation is observed specifically at 10-5 M in HemSCs. Propranolol hydrochloride (10-9 M-10-3 M; 24 and 48 hours) significant decreases cell proliferation at 10-4 M propranolol after 24 hours and 10-9 M propranolol after 48 hours in HemSCs. Propranolol hydrochloride (50 μM-200 μM; 24 hours) increases Annexin V positivity and caspase-3 activation, rapidly induces HemSC apoptosis. Western blot analysis. Cell line: HemSC cells. Concentration: 10-7 M-10-3 M. Incubation time: 24 and 48 hours. Result: Increased the total ERK1/2 levels in a dose-dependent manner. Cell Viability Assay Cell line: HemSC cells. Concentration: 10-9 M-10-3 M. Incubation time: 24 and 48 hours. Result: Suppressed HemSC Proliferation. Apoptosis Analysis Cell line: HemSC cells. Concentration: 50 μM, 100 μM, or 200 μM. Incubation time: 24 hours. Result: Induced HemSC cell death occurred via an apoptotic pathway.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ICI 45520 | NSC 91523

Quick Database Links

Gene Symbol

Adrenergic Receptor