Prol1 Antibody

• Catalog Number: orb1249350
• Category: Antibodies
• Description: Prol1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Mouse
• Immunogen: The immunogen for this antibody is: C-QFPVRKYLEDPRY
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 45kDa band in Mouse Eye lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.6kDa according to NP_032670.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:Positive staining in the submandibular salivary gland of the mouse, while cells remain negative in the sublingual salivary gland. Data provided by Everest Grant winner Melinda Larsen State University of New York, Albany, NY. A publication from this author includes the use of this antibody: Nelson at al, Biol Open. 2013 Apr 18;2(5):439-47, PMID: 23789091.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Prol1
• UniProt ID: E9PYQ4
• NCBI: NP_032670.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Prol1, proline rich, lacrimal 1, Muc10, mucin 10,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 45kDa band in Mouse Eye lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.6kDa according to NP_032670.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:Positive staining in the submandibular salivary gland of the mouse, while cells remain negative in the sublingual salivary gland. Data provided by Everest Grant winner Melinda Larsen State University of New York, Albany, NY. A publication from this author includes the use of this antibody: Nelson at al, Biol Open. 2013 Apr 18;2(5):439-47, PMID: 23789091.
• Expiration Date: 12 months from date of receipt.

orb1249350 (2.9 ug/ml) staining of cells in the submandibular salivery gland (SMG), but not in the sublingual salivery gland (SLG) in mouse.