PPAR Gamma 2 antibody

• Catalog Number: orb345410
• Category: Antibodies
• Description: PPAR Gamma 2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human PPAR gamma 2.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:4,000 - 1:40,000, WB: 1:1,000 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human PPAR gamma isoform 2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and macaque based on 100% homology for the immunogen sequence. Cross reactivity with PPARg2 protein from dog and swine is likely due to 94% homology (15/16 identities) with the protein from these sources. Cross reactivity does occur with PPARg2 from mouse. Mouse PPARg2 shows 87% homology to the immunogen. Cross reactivity with PPARg2 homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
• Conjugation: Unconjugated
• UniProt ID: P37231
• NCBI: 20336229
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-PPAR gamma 2 antibody, Peroxisome prol
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a single band approximately 55 kDa in size corresponding to PPARg2 by western blot in the appropriate tissue or cell lysate.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of mouse 3T3 whole cell lysate using PPAR Gamma 2 antibody