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POMC Antibody / ACTH / N-Terminal

Catalog Number: orb2640330

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2640330
CategoryAntibodies
DescriptionACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
Species/HostMouse
ClonalityRecombinant
Clone Numberr57
Tested applicationsELISA, IHC-P
ReactivityHuman, Mouse, Rat
IsotypeMouse IgG1, kappa
ImmunogenAn N-terminal peptide (within AA 1-24) from the Synacthen/ACTH region of human POMC was used as the immunogen for this recombinant ACTH antibody.
Antibody TypePrimary Antibody
Dilution rangeELISA (order BSA/sodium azide-free format for coating),Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis recombinant ACTH antibody is available for research use only.
UniProt IDP01189
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe optimal dilution of the recombinant ACTH antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
POMC Antibody / ACTH / N-Terminal

IHC testing of FFPE human pituitary gland with recombinant ACTH antibody (clone r57). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

POMC Antibody / ACTH / N-Terminal

SDS-PAGE analysis of purified, BSA-free recombinant ACTH antibody (clone r57) as confirmation of integrity and purity.

  • POMC Antibody / ACTH / N-Terminal [orb385765]

    ELISA,  FACS,  IF,  IHC-P

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • POMC Antibody / ACTH / N-Terminal [orb2639871]

    ELISA,  FACS,  IF,  IHC-P

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • POMC Antibody / ACTH / N-Terminal [orb386195]

    ELISA,  IHC-P

    Human, Mouse, Rat

    Mouse

    Recombinant

    Unconjugated

    20 μg