Polyphyllin VI

SKU: orb1222038

Description

Polyphyllin VI has hemostasis , expectorant , bacteriostasis, anticytotoxic, anti pregnancy kill sperm effects.

Key Properties

• CAS Number: 55916-51-3
• MW: 738.91
• Purity: >98% (HPLC)
• Formula: C₃₉H₆₂O₁₃
• SMILES: CC1CCC2(C(C3(C(O2)CC4C3(CCC5C4CC=C6C5(CCC(C6)OC7C(C(C(C(O7)CO)O)O)OC8C(C(C(C(O8)C)O)O)O)C)C)O)C)OC1
• Target: Telomerase
• Solubility: In Vitro: DMSO : 100 mg/mL (135.34 mM)

Bioactivity

• In Vivo:
  Animal model: A549 tumor xenografts subcutaneously inoculated into the right flank of the nude mice. Dosage: 2 mg/kg, 3 mg/kg, 4 mg/kg. Administration: Intraperitoneal Injection (i.p.). Result: Resulted the reduction of tumor volume to 25.63%, 41.71%, and 40.41%, respectively, after 2 mg/kg, 3 mg/kg and 4 mg/kg treatment. Animal model: A549 tumor xenografts subcutaneously inoculated into the right flank of the nude mice. Dosage: 2.5 mg/kg, 5 mg/kg, 10 mg/kg. Administration: Intraperitoneal Injection (i.p.). Result: Showed the expression of NLRP3, caspase-1, IL-1β and GSDMD was increasing in a dose manner.
• In Vitro:
  Cell Viability Assay Cell line: A549, NCI-H1299, and HepaRG cells. Concentration: 0 μM, 2.5 μM, 5.0 μM, 7.5 μM, 10 μM, 12.5 μM (A549, NCI-H1299), 0 μM, 2 μM, 4.0 μM, 6.0 μM, 8.0 μM, 10.0 μM, 12.0 μM, 16.0 μM (HepaRG) Incubation time: 48 h (A549, NCI-H1299), 24 h and 48 h (HepaRG). Result: Showed the IC50 value in NCI-H1299 cells after 48h treatment was 1.87±0.09 μM, 1.59±0.12 μM in A549.Demonstrated the reduction of HepaRG cell viability ranged from 88.90% to 1.07% after 24 h, and from 79.06% to 0.71% after 48 h. Cell Viability Assay Cell line: A549, NCI-H1299 cells. Concentration: 0.5 μM, 1.0 μM, 2.0 μM Incubation time: 24 h. Result: Showed those of A549 cells in G2/M phase were 25.14%±3.31%, 28.40%±4.63%, and 42.66%±1.30%, and NCI-H1299 cells were 27.99%±4.68%, 30.24%±3.61% and 38.51%±5.10% after treatment with 0.5, 1, and 2 μM for 24 h, respectively. Cell Cycle Analysis Cell line: HepaRG cells. Concentration: 0 μM, 2.0 μM, 4.0 μM, 6.0 μM, 8.0 μM, 12.0 μM Incubation time: 24 h. Result: Resulted the ratios of cells in the S and G0/G1 phase changed from 23.62%±0.14% to 34.01%±0.32%, 66.88%±1.15% to 54.00%±0.71%, respectively. Western blot analysis. Cell line: A549, NCI-H1299 cells. Concentration: 0 μM, 3 μM, 4 μM, 5 μM, 6 μM. Incubation time: 24 h. Result: Resulted dose-dependently increasing the protein expression of NLRP3 and ASC, and the cleaved form of caspase-1, IL-1β, IL-18 and GSDMD in A549 and NCI-H1299 cells. Immunofluorescence Cell line: A549, NCI-H1299 cells. Concentration: 0 μM, 3 μM, 4 μM, 5 μM, 6 μM. Incubation time: 24 h. Result: Resulted the expression of caspase-1 was significantly inhibited by N-acetyl-L-cysteine (NAC) in Polyphyllin VI -treated A549 and NCI-H1299 cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Telomerase