PolQi2

SKU: orb2283464

Description

PolQi2 is a small molecule inhibitor of DNA polymerase theta (Polθ) that specifically suppresses its helicase activity. When combined with the DNA-PK inhibitor AZD7648, it enhances homology-directed repair (HDR) efficiency and improves the accuracy of genome editing in cellular models.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 2565638-16-4
• MW: 453.9
• Formula: C₂₁H₁₆ClN₅O₃S
• SMILES: C(NC=1SC(OCC2=CC=C(Cl)C=N2)=NN1)(=O)C=3C(=CN=CC3)C4=C(OC)C=CC=C4
• Target: Others
• Solubility: DMSO:155 mg/mL (341.48 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (7.27 mM)

Bioactivity

• In Vitro:
  PolQi2 is a DNA polymerase θ (Pol generative) inhibitor that targets the inhibition of ALT-EJ (alternative end-joining) repair by inhibiting the Pol generative N-terminal helicase domain. The combination of PolQi2 (3 μM, 14 days) and DNA-PK (1 μM, 14 days) inhibitors can efficiently and stably achieve targeted gene insertion in HeLa and Jurkat cells without adversely affecting cell proliferative activity.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Compound Identifiers

PubChem CID

155353937