PLK1 Antibody / Polo like kinase 1

• Catalog Number: orb1825344
• Category: Antibodies
• Description: Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and CDC5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle; Plk protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant fragment of human protein (within amino acids 1-200) was used as the immunogen for the Polo like kinase 1 antibody.
• UniProt ID: P53350
• Tested applications: ELISA, IHC-P
• Dilution range: ELISA (Order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Antibody Type: Primary Antibody
• Clone Number: PLK1/7122
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Polo like kinase 1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human tonsil tissue with Polo like kinase 1 antibody (clone PLK1/7122). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with Polo like kinase 1 antibody (clone PLK1/7122). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node tissue with Polo like kinase 1 antibody (clone PLK1/7122). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.