PRKCE Antibody

SKU: orb1263534

Description

PRKCE Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Reactivity: Human
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This PKC epsilon antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the N-terminal region of human PKC epsilon.
• Target: PRKCE
• Molecular Weight: 84 kDa
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein kinase C epsilon type, nPKC-epsilon, PRKCE, PKCE

Western blot analysis of anti-PKCepsilon Pab in placenta lysate. PKCepsilon was detected using purified Pab.

Western blot analysis of PRKCE using rabbit polyclonal PKC epsilon Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCE gene (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

PRKCE

UniProt

Q02156

RefSeq:Q02156