Pimobendan

SKU: orb1307669

Description

Pimobendan (UD-CG 115 BS) is a cardiotonic agent that selectively inhibits phosphodiesterase 3 (PDE3) with an IC50 of 0.32 µM. It is used in cardiovascular research to study inotropic and vasodilatory effects, with applications in both in vitro enzymatic assays and in vivo models of heart failure.

Research Area

Metabolism Research

Key Properties

• CAS Number: 74150-27-9
• MW: 334.37
• Purity: 99.91% (May vary between batches)
• Formula: C₁₉H₁₈N₄O₂
• SMILES: CC1C(C=2C=C3C(N=C(N3)C4=CC=C(OC)C=C4)=CC2)=NNC(=O)C1
• Target: PDE
• Solubility: Ethanol:5 mg/mL (14.95 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:55 mg/mL (164.49 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.98 mM)

Bioactivity

• Target IC50:
  PDE3:0.32 μM
• In Vivo:
  When administered to human atrial cells, Pimobendan (100 μM) increased the L-type calcium current by 250.4% (EC50: 1.13 μM). Its effect on rabbit atrial cells was significantly lower, resulting in a 67.4% increase in ICa(L). Pimobendan exhibits selective inhibitory action on isolated PDE III in guinea pig myocardium (IC50: 0.32 μM), with much higher IC50s for PDE I and PDE II (>30 μM). It also inhibits cAMP-PDE III activity (IC50: 2.4 μM) and induces a positive inotropic effect on isolated guinea pig papillary muscles (EC50: 6.0 μM), partially due to its selective inhibition of myocardial PDE III.
• In Vitro:
  Compared to the control group, Pimobendan (1 mg/kg) significantly reduced the permeability of cardiomyocytes and also decreased the levels of TNF-α and IL-1β within the chamber without affecting myocardial nerves, heart weight, and body weight. In a mouse model of EMC virus-induced myocarditis, Pimobendan contributed to an extension of lifespan. The final survival rate was significantly increased from 33.6% (control group) to 53.3% (0.1 mg/kg) or 66.7% (1 mg/kg). Additionally, Pimobendan inhibited the expression of inducible nitric oxide synthase genes in the heart, thereby reducing the content of nitric oxide within the heart.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UD-CG 115, UD-CG 115 BS, UD-CG115, UD-CG-115, Pimobendane, Pimobendan, Phosphodiesterase (PDE), phosphodiesterase, Acardi, inhibit, PDE3, Inhibitor

Compound Identifiers

PubChem CID

4823