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PIK3C2A Antibody
SKU: orb1255783
Featured
Description
Research Area
Cell Biology, Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PIK3C2A (NP_002636.2). |
| Target | PIK3C2A |
| Molecular Weight | Observed: 191kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−PIK3C2A, CPK, PI3-K-C2A, PI3K-C2alpha, PI3-K-C2(ALPHA), phosphoinositide-3-kinase, class 2, alpha polypeptide, C2-containing phosphatidylinositol kinase
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Western blot analysis of extracts of various cell lines, using PIK3C2A antibody (orb1255783) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse heart using PIK3C2A antibody (orb1255783) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using PIK3C2A antibody (orb1255783) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using PIK3C2A antibody (orb1255783) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using PIK3C2A antibody (orb1255783) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug PIK3C2A antibody (orb1255783). Western blot was performed from the immunoprecipitate using PIK3C2A antibody (orb1255783) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
PIK3C2A
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
PIK3C2A Antibody (orb1255783)
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