PIK3C2A Antibody

• Catalog Number: orb1255783
• Category: Antibodies
• Description: PIK3C2A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PIK3C2A (NP_002636.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 191kDa
• Target: PIK3C2A
• UniProt ID: O00443
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PIK3C2A, CPK, PI3-K-C2A, PI3K-C2alpha, PI3-K-C2(AL
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PIK3C2A antibody (orb1255783) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse heart using PIK3C2A antibody (orb1255783) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using PIK3C2A antibody (orb1255783) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver damage using PIK3C2A antibody (orb1255783) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using PIK3C2A antibody (orb1255783) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HeLa cells, using 3 ug PIK3C2A antibody (orb1255783). Western blot was performed from the immunoprecipitate using PIK3C2A antibody (orb1255783) at a dilution of 1:1000.