Phospho-TNIK(S764) Antibody

• Catalog Number: orb1925241
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This TNIK Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S764 of human TNIK.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9UKE5
• MW: 154943 Da
• Tested applications: DOT, IHC-P, WB
• Dilution range: IHC-P: 1:50~100, DB: 1:500
• Antibody Type: Primary Antibody
• Clone Number: RB7150
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: TRAF2 and NCK-interacting protein kinase, TNIK, KI
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_001155033.1, NP_001155034.1, NP_001155037.1, NP_001155038.1, NP_001155036.1, NP_001155035.1, NP_001155032.1, NP_055843.1
• Expiration Date: 12 months from date of receipt.

Dot blot analysis of Phospho-TNIK-pS764 Pab and TNIK-pS764 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml. P-Pab: phosphorylated antibody; NP-Pab: non-phophorylated antibody; P-Pep: phospho-peptide; NP-Pep: non-phospho-peptide.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.