Phospho-RAD9(S328) Antibody

• Catalog Number: orb1931236
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This RAD9 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S328 of human RAD9.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q99638
• MW: 42547 Da
• Tested applications: DOT, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, DB: 1:500
• Antibody Type: Primary Antibody
• Clone Number: RB7115
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cell cycle checkpoint control protein RAD9A, hRAD9
• Note: For research use only
• NCBI: NP_004575.1, NP_001230153.1

Dot blot analysis of anti-Phospho-Rad9-S328 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-Phospho-Rad9-S328 Pab is used in Western blot to detect Phospho-Rad9-S328 in HL60 (left) and Y79 (right) tissue lysates.