Phospho-PDHE1A(S232) Antibody

• Catalog Number: orb1931033
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PDHE1A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S232 of human PDHE1A.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P08559
• MW: 43296 Da
• Tested applications: DOT, WB
• Dilution range: WB: 1:500, WB: 1:5000, DB: 1:500
• Clone Number: RB29473
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Pyruvate dehydrogenase E1 component subunit alpha,
• Research Area: Cancer Biology, Cell Biology, Metabolism Research,
• Note: For research use only
• NCBI: NP_001166925.1, NP_001166926.1, NP_000275.1, NP_001166927.1

Dot blot analysis of anti-mPDHE1A-S232 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Western blot analysis of lysates from C6 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A (S232) Antibody (upper) or Tubulin (lower).

Western blot analysis of lysates from 293 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A (S232) Antibody (upper) or Tubulin (lower).