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Item 1 of 3
Phospho-PDHE1A(S232) Antibody
Catalog Number: orb1931033
| Catalog Number | orb1931033 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This PDHE1A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S232 of human PDHE1A. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Porcine, Rat |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | P08559 |
| MW | 43296 Da |
| Tested applications | DOT, WB |
| Dilution range | WB: 1:500, WB: 1:5000, DB: 1:500 |
| Clone Number | RB29473 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Pyruvate dehydrogenase E1 component subunit alpha, Read more... |
| Note | For research use only |
| NCBI | NP_001166925.1, NP_001166926.1, NP_001166927.1, NP_000275.1 |
Dot blot analysis of anti-mPDHE1A-S232 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Western blot analysis of lysates from C6 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A (S232) Antibody (upper) or Tubulin (lower).
Western blot analysis of lysates from 293 cell line, untreated or treated with CIP, 100ng/ml, using Phospho-PDHE1A (S232) Antibody (upper) or Tubulin (lower).
