PHF6 Antibody (C-Term)

• Catalog Number: orb1925886
• Category: Antibodies
• Description: PHF6 Antibody (C-Term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q8IWS0
• MW: 41290 Da
• Tested applications: IF, WB
• Dilution range: IF - 1:25, WB - 1:2000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CENP-31, KIAA1823
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PHF6 Antibody (C-Term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PHF6 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).