Periostin antibody

SKU: orb2276307

Description

Periostin antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Skin: A strong periostin immunostaining should be seen in the skin, forming a subepithelial band of variable thickness. Negative Control: Skin: Periostin immunostaining should be lacking in epithelial cells. Cellular Localization: Extracellular space Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: MSVA-649M
• Immunogen: Recombinant fragment (around aa193-326) of human POSTN protein (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Fasciclin-I like; Osteoblast specific factor 2 (OSF2); PDLPOSTN; Periodontal ligament specific periostin; Periostin isoform thy2 / th4 / thy6 / thy8; Periostin osteoblast specific factor; PN; POSTN

Abundant periostin positive stroma in an invasive lobular breast cancer.

Rectum mucosa A periostin positive subepithelial band is regularly seen in the colorectal mucosa. Additional periostin deposits occur in the lamina propria.

Skin Periostin is most regularly seen in a subepithelial band of variable thickness in the skin.

Quick Database Links

UniProt

Q15063