PDP1 Recombinant Rabbit Monoclonal Antibody

SKU: orb1172478

Description

PDP1 Recombinant Rabbit Monoclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:500-1000, ICC/IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG/Kappa
• Clone No.: F12B3
• Target: PDP1
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PDH; PDP; PDPC; PDPC 1; PPM2A; PPM2C; Gm1024; PDP1_HUMAN; PDP1; PDP 1; Protein phosphatase 2C; Pyruvate dehydrogenase phosphatase catalytic subunit 1 (PDPC 1); 3.1.3.43; PDP1_MOUSE; PDP1_RAT;

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: U87MG, SK-OV-3, PC-3, 3T3, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 55 kDa, Observed MW: 55 kDa.

Cell line: U87-MG, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172478.

Quick Database Links

Gene Symbol

PDP1