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PDIA3 Antibody (Center)
Catalog Number: orb1787955
| Catalog Number | orb1787955 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | 192-220 aa |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Conjugation | Unconjugated |
| MW | 56782 Da |
| Target | This PDIA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-220 amino acids from the Central region of human PDIA3. |
| UniProt ID | P30101 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Protein disulfide-isomerase A3, 58 kDa glucose-reg Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of widr cells using PDIA3 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-PDIA3 Antibody (Center) at 1:1000 dilution. Lane 1: A2058 whole cell lysate. Lane 2: A375 whole cell lysate. Lane 3: Ramos whole cell lysate. Lane 4: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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