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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, mouse IgG1 Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human PD-1, His Tag & Fc tag with a linear range of 0.05-0.2 ug/mL (Routinely tested). |
| Target | PD-L1 |
| Tag | Mouse Fc Tag |
| MW | 51.6 kDa |
| Purity | >92% as determined by SDS-PAGE.Endotoxin level is less than 0.1 EU per ug by the LAL method. |
| Protein Sequence | Phe 19 - Arg 238 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immobilized Human PD-L1, mouse IgG1 Fc Tag at 5 ug/ml (100 uL/well) can bind Biotinylated Human PD-1, His Tag & Fc tag with a linear range of 0.05-0.2 ug/ml.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_054862.1 |
|---|
Documents Download
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Protocol Information
PD-L1 Recombinant Protein (orb1227730)
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