PD-1 Antibody [J43], Rabbit IgG

• Catalog Number: orb1671671
• Category: Antibodies
• Description: PD-1 Antibody [J43], Rabbit IgG
• Target: Pdcd1
• Clonality: Recombinant
• Isotype: IgG lambda
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: batch dependent
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: This antibody was raised by immunising Armenian hamsters with B12 cells, a PD-1 cDNA transfectant of BHK cells.
• UniProt ID: Q02242
• Tested applications: Depletion, ELISA, FC, IHC, IP, NeA
• Antibody Type: Primary Antibody
• Clone Number: J43
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD279, PD1, PD 1, Programmed cell death protein 1,
• Note: For research use only

Flow cytometry using the Anti-PD-1 antibody J43. Paraformaldehyde fixed mouse splenocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of J43 (orb1671671 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes with anti-PD-1 (J43). Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of J43 (orb1671671) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671671- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using Anti-PD-1 antibody J43. Mouse lymph node tissue lysate (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of J43 (orb1671671) at 0.003 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Binding curve of anti-PD-1 antibody J43 (orb1671671) to recombinant mouse PD-1 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse PD-1 Fc-Fusion Protein (Pr00152-1.9; Antibody) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 ng/ml was performed using orb1671671. For detection- a 1:4000 dilution of HRP-lnti-ribody was used.