PCMT1 Recombinant Mouse Monoclonal Antibody

SKU: orb1172962

Description

PCMT1 Recombinant Mouse Monoclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:500-1000, ICC/IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG1, Kappa
• Clone No.: C8C7
• Target: PCMT1
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PIMT; PCM; PIMT_HUMAN; PCMT1; L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase; 2.1.1.77; PIMT_MOUSE; PIMT_RAT;

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: HeLa, 2: 293T, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 27 kDa, Observed MW: 27 kDa.

Cell line: HeLa, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172962.

Quick Database Links

Gene Symbol

PCMT1