PAMP-12 (human, mouse, rat, porcine, bovine) TFA

SKU: orb1980104

Description

Proadrenomedullin N-terminal 12 peptide (PAMP-12), an endogenous fragment originating from the adrenal medulla and spanning amino acids 9-20 of human PAMP-20, plays a role in causing hypotension. It functions as an agonist for the MAS-related G protein-coupled receptor family member X2 (MRGPRX2), inhibiting forskolin-stimulated cAMP accumulation in CHO cells expressing human MRGPRX2 (EC50 = 57.2 nM) and selectively prompting calcium mobilization in these cells (EC50 = 41 nM), but not in cells expressing MRGPRX1, MRGPRX3, or MRGPRX4 at 1 µM. As an antagonist of nicotinic acetylcholine receptors (nAChRs), PAMP-12 obstructs carbachol-triggered catecholamine release and the influx of calcium and sodium (IC50s = 1.3, 0.39, and 0.87 µM, respectively) in primary bovine adrenal chromaffin cells, with no effect on histamine-induced responses (IC50s = >1 µM for all). Additionally, it decreases mean arterial blood pressure in normotensive rats at doses ranging from 10 to 50 nmol/kg.

Key Properties

• MW: 1618.93
• Formula: C₇₇H₁₁₉N₂₅O₁₄.XCF₃COOH
• SMILES: N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCCN)C(N[C@@H](CCCCN)C(N[C@H](C(N[C@@H](CC(N)=O)C(N[C@@H](CCCCN)C(N[C@H](C(N[C@@H](C)C(N[C@@H](CC(C)C)C(N[C@@H](CO)C(N[C@H](C(N)=O)CCCNC(N)=N)=O)=O)=O)=O)CC2=CNC3=CC=CC=C23)=O)=O)=O)CC4=CNC5=CC=CC=C45)=O)=O)=O)=O.OC(C(F)(F)F)=O

Storage & Handling

• Storage: -20°C
• Disclaimer: For research use only