PAK6 Antibody (Center)

• Catalog Number: orb1937697
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PAK6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-297 amino acids from the Central region of human PAK6.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9NQU5
• MW: 74869 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB31991
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Serine/threonine-protein kinase PAK 6, PAK-5, p21-
• Research Area: Immunology & Inflammation, Signal Transduction
• Note: For research use only
• NCBI: NP_001263646.1, NP_001122100.1, NP_001263647.1, NP_064553.1, NP_001122101.1
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PAK6 Antibody (Center) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HT-29 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 75 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of PAK6 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

PAK6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PAK6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

PAK6 Antibody (Center) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the PAK6 antibody detected the PAK6 protein (arrow).