RPS6KB1 Antibody

SKU: orb1258229

Description

RPS6KB1 Antibody

Research Area

Cell Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human P70 S6K (NP_003152.1).
• Target: RPS6KB1
• Molecular Weight: Observed: 70kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RPS6KB1, ribosomal protein S6 kinase, 70kD, polypeptide 1, PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha, p70 S6 kinase, alpha 1, alpha 2, serine/threonine kinase 14 alpha

Western blot analysis of extracts of various cell lines, using P70 S6K antibody (orb1258229) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of C6 cells using P70 S6K antibody (orb1258229) at dilution of 1:100. C6 cells were treated by Serum-starvation overnight at 37 ℃. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using P70 S6K antibody (orb1258229) at dilution of 1:100. NIH/3T3 cells were treated by Serum-starvation overnight at 37 ℃. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

RPS6KB1

UniProt

P23443