p27K1 Antibody

SKU: orb749347

Description

Mouse monoclonal antibody against p27 conjugated to CF488

Images & Validation

• Tested Applications: FACS, IF
• Dilution Range: Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1-2ug/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the p27K1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: SX53G8
• Immunogen: Purified GST-p27 fusion protein of human origin was used as the immunogen for this p27Kip1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: CF488

Storage & Handling

• Storage: Store the p27K1 antibody at 2-8°C, protected from light.
• Buffer/Preservatives: 500 ul at 0.1 mg/ml with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Intracellular FACS testing of human HeLa cells with p27Kip1 antibody (green), isotype control (gray), and cells without primary (black).