OTOP1 Antibody (Center)

SKU: orb1937808

Description

Research Area

Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	IHC-P, WB
Dilution range	WB - 1:2000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	67353 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

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All lanes: Anti-OTOP1 Antibody (Center) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Human heart whole tissue lysate. Lane 3: Mouse kidney whole tissue lysate. Lane 4: Rat kidney whole tissue lysate. Lane 5: HACAT whole cell lysate. Lane 6: Mouse heart whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-OTOP1 Antibody (Center) at 1:1000 dilution. Lane 1: HACAT whole cell lysate. Lane 2: A431 whole tissue lysate. Lane 3: Mouse kidney whole tissue lysate. Lane 4: Mouse heart whole tissue lysate. Lane 5: Rat kidney whole cell lysate. Lane Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

OTOP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of OTOP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

UniProt

Q7RTM1

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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