O-Propargyl-puromycin-10 %

SKU: orb1733750

Description

Liu et al.[1] reported a non-radioactive alternative to analyze newly synthesized proteins in cell culture and whole organisms that is based on O-Propargyl-puromycin, an alkyne analog of puromycin.\nO-Propargyl-puromycin is cell-permeable and incorporates into the C-terminus of translating polypeptide chains thereby stopping translation.\nThe resulting truncated C-terminal alkyne labeled proteins can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.\nIn contrast to Azidohomoalanine (AHA) or Homopropargylgycine (HPG) based non-radioactive methionine analog-approaches, methionine free-medium is not required for O-Propargyl-purmoycin-based monitoring of nascent protein synthesis.\nPresolski et al.[4] and Hong et al.[5] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.

Images & Validation

• Application Notes:
  Protein synthesis monitoring in cell culture and whole organisms[1,2], Spectroscopic Properties: λmax 275 nm, ε 20.0 L mmol-1 cm-1

Key Properties

• CAS Number: 1416561-90-4
• MW: 495.53 g/mol (free amine)
• Purity: ≥ 95 % (HPLC)
• Formula: C₂₄H₂₉N₇O₅ (free amine)
• SMILES: N(c1ncnc2c1ncn2[C@@H]1O[C@@H]([C@@H](NC(=O)[C@H](Cc2ccc(cc2)OCC#C)N)[C@H]1O)CO)(C)C
• Solubility: DMSO, PBS (up to 50 mM tested) pH adjusted to 5.0

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Acetate salt

Compound Identifiers

PubChem CID

71576433