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Catalog Number | orb1938512 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB28179 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:500, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 18609 Da |
Target | This NUDT15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-118 amino acids from the C-terminal region of human NUDT15. |
UniProt ID | Q9NV35 |
NCBI | NP_060753.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Probable 8-oxo-dGTP diphosphatase NUDT15, 8-oxo-dG Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-NUDT15 Antibody (C-term) at 1:1000 dilution + HuTu80 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-NUDT15 Antibody (C-term) at 1:1000 dilution + SW620 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-NUDT15 Antibody (C-term) at 1:500 dilution + Mouse small intestine tissue lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
NUDT15 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NUDT15 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NUDT15 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.