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NSUN6 Antibody
Description
Images & Validation
−| Tested Applications | IF, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 220-469 of human NSUN6 (NP_872349.1). |
| Target | NSUN6 |
| Molecular Weight | Observed: 52kDa |
| Purification | Affinity purification |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−NSUN6 Rabbit Polyclonal Antibody [orb312329]
IF, IHC-Fr, IHC-P, WB
Human
Rabbit
Polyclonal
Unconjugated
200 μl, 100 μl, 50 μlNSUN6 Antibody [orb341254]
IF, WB
Human, Mouse
Rabbit
Polyclonal
Unconjugated
200 μl, 100 μl, 50 μl, 30 μl

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Western blot analysis of extracts of various cell lines, using NSUN6 antibody (orb1260414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunofluorescence analysis of MCF-7 cells using NSUN6 antibody (orb1260414). Blue: DAPI for nuclear staining.
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Protocol Information
NSUN6 Antibody (orb1260414)
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