NRK Antibody (Center)

SKU: orb1936680

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:10~50, WB: 1:1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB32669
• Target: This NRK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 721-750 amino acids from the Central region of human NRK.
• Molecular Weight: 178479 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nik-related protein kinase, NRK

Fluorescent confocal image of Hela cell stained with NRK Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NRK immunoreactivity is localized to Cytoplasm and Nucleus significantly.

NRK Antibody (Center) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the NRK antibody detected the NRK protein (arrow).

Quick Database Links

Gene Symbol

This NRK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 721-750 amino acids from the Central region of human NRK.

UniProt

Q7Z2Y5

RefSeq (Protein):NP_940867.2