NQ301

SKU: orb1303791

Description

NQ301 is a small molecule antithrombotic agent that potently inhibits collagen-induced platelet aggregation in rabbit models with an IC50 of 10 mg/mL. It is a useful research tool for studying hemostasis and thrombosis mechanisms in both in vitro and in vivo experimental settings.

Research Area

Protein Biochemistry

Key Properties

• CAS Number: 130089-98-4
• MW: 325.75
• Purity: 99.81% (May vary between batches)
• Formula: C₁₈H₁₂ClNO₃
• SMILES: CC(=O)c1ccc(NC2=C(Cl)C(=O)c3ccccc3C2=O)cc1
• Target: Thrombin,Platelet aggregation
• Solubility: DMSO:6.88 mg/mL (21.12 mM)

Bioactivity

• Target IC50:
  Platelet aggregation (rabbit, collagen-challenged):10 mg/mL
• In Vitro:
  NQ301 dose-dependently inhibits collagen (10 mg/mL, IC50: 0.60±0.02 μM), U46619 (1 mg/mL, IC50: 0.58±0.04 μM) and arachidonic acid (100 mg/mL, IC50: 0.78±0.04 μM) challenged rabbit platelet aggregation. NQ301 potently suppresses thromboxane B2 formation by platelets that are exposed to arachidonic acid in a concentration-dependent manner, but had no effect on the production of prostaglandin D2, indicating an inhibitory effect on thromboxane A2 synthase. NQ301 markedly inhibits the increase of cytosolic Ca2+ concentration and ATP secretion, and also markedly increases platelet cAMP levels in the activated platelets. The antiplatelet activity of NQ301 may be mediated by inhibition of cytosolic Ca2+ mobilization, enhancement of cAMP production and inhibition of ATP secretion in activated platelets.
• Cell Research:
  ished rabbit platelet suspension is challenged by addition of collagen (10 mg/mL), arachidonic acid (100 μM) or U46619 (1 μM). Concentration- response relationship is determined in the absence or presence of a range of concentrations of NQ301 (0, 0.25, 0.5, 0.75, 1 μM); aspirin-treated platelets (50 μM for 5 min) are used to prevent any possible contribution of endogenous arachidonic acid metabolites to platelet aggregation. The resulting aggregation, measured as the change in light transmission, is recorded for 5 min. The extent of platelet aggregation is expressed as % of the control.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, NQ 301, NQ301, NQ-301, inhibit, Platelet aggregation, Plateletaggregation, Thrombin

Compound Identifiers

PubChem CID

781109