NpFR1

SKU: orb1714148

Description

Reversible fluorescence intensity-based redox sensor

Key Properties

• MW: 431.45
• Formula: C₂₃H₂₁N₅O₄
• SMILES: C1(N(C(C2=CC5=C(C3=CC=CC1=C23)N(C4=NC(NC(C4=N5)=O)=O)CCC)=O)CCCC)=O
• Solubility: Soluble in DMSO

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Hazard Information: Classification: Caution: Substance not yet fully tested. Safety Phrases: S22 - Do not breathe dust S24/25 - Avoid contact with skin and eyes S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection
• Disclaimer: For research use only

Fluorescence behavior of NpFR1 in the oxidized (black) and reduced (red) forms, using 50 μM. Excitation: 405 or 488 nm (not shown). Emission: 490 – 600 nm, with peak at 545 nm.

Chemical structure of NpFR1, a reversible fluorescence intensity-based redox sensor.

Fluorescence emission of NpFR1 (5 mM, lex = 405 nm) with the incremental addition of sodium dithionite. All spectra were acquired in HEPES buffer (100 mM, pH 7.4).

Imaging of NpFR1 in 3T3-L1 preadipocytes treated with (a) vehicle control (b) H2O2 (100 mM, 2 min), (c) NpFR1 (50 mM, 2 h) and (d) NpFR1 (50 mM, 2 h) followed by H2O2 (100 mM, 2 min). Scale bar represents 50 mm, lex = 405 nm. (e) Integrated emission from 510 nm to 610 nm. Values are the mean ratio generated from the intensity from five fields of cells. Error bars represent standard error measurement (s.e.m.).

Fluorescence response of NpFR1 to cycles of oxidation and reduction. Reduction was achieved with sodium dithionite (100mM) followed by re-oxidation with 250mM H2O2. Spectra were recorded 5 min after the addition of reducing and oxidising agents. All spectra were acquired in HEPES buffer (100 mM, pH 7.4).

Compound Identifiers

PubChem CID

102143041