Nomilin

SKU: orb1303925

Description

Nomilin

Research Area

Signal Transduction

Key Properties

• CAS Number: 1063-77-0
• MW: 514.56
• Purity: 99.36%
• Formula: C₂₈H₃₄O₉
• SMILES: CC(=O)OC1CC(=O)OC(C)(C)C2CC(=O)C3(C)C(CCC4(C)C(OC(=O)C5OC345)c3ccoc3)C12C
• Target: MAPK
• Solubility: H2O:Insoluble;10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.89 mM);Ethanol:Insoluble;DMSO:125 mg/mL (242.93 mM)

Bioactivity

• In Vivo:
  Nomilin is an effective inducer of gluthathione S-transferase activity in mice and to inhibit carcinogenesis in the hamster buccal pouch assay. Nomilin can shorten anaesthetic-induced sleeping time in mice, probably through a stimulant action o the central nervous system. Nomilin significantly inhibited tumour-directed capillary formation. Serum proinflammatory cytokines such as IL-1β, IL-6, TNF-α and GM-CSF and also serum NO levels were significantly reduced b the treatment of nomilin. Administration of Nomilin significantly reduce the serum level of VEGF, a proangiogenic factor and increase the antiangiogenic factors TIMP-1 and IL-2.
• In Vitro:
  Administration of Nomilin significantly retarded endothelial cell proliferation, invasion, migration and tube formation. It also possesses anti Proliferative activity agains the number of human cancer cell lines including leukemia (HL-60), ovary (SKOV-3), liver (Hep G2), cervix (HeLa), stomach (NCI-SNU-1), and breast (MCF-7. Nomilin significantly decreased TRAP-positive multinucleated cell numbers compared wit the control and exhibited no cytotoxicity. It decreases bone resorption activity and downregulates osteoclast-specific genes, NFATc1 and TRAP mRNA levels. furthermore , Nomilin suppressed MAPK signaling pathways. Thus, Nomilin has inhibitory effects on osteoclastic differentiation in vitr.
• Cell Research:
  Cell lines: HUVECs. concentration : 5 μg-500 μg/ml. Incubation Time: 48 h. Method:. HUVECs were seeded (5000 cells/well) in 96-well flat-bottomed titer plate and incubated for 24 h at 37℃n 5% CO2 atmosphere. Different concentration of Nomilin (5 μg-500 μg/ml) were added and incubated further for 48 h. Before 4 h completion of incubation, 20 μL MTT (5 mg/mL) was added. Percentage of dead cells was determined using an ELISA plate reader set to record absorbance at 570 nM.
• Animal Research:
  Animal Models: Four to Six week old Male C57B-/- mice. Formulation: light paraffin oil. Dosages: 6 mg/kg, I.P.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

compound, anti-hyperglycemic, anti-obesity, Inhibitor, MAPK, ischemia-reperfusion, inhibit, Nomilin, limonoid

Compound Identifiers

PubChem CID

326240