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Grin2b Antibody

SKU: orb1273486

Description

Grin2b Antibody

Research Area

Neuroscience

Images & Validation

Tested ApplicationsIHC, IP, WB
ReactivityHuman, Rat
Application Notes
NMDA NR2B Subunit antibody is specific for the 180 kDa NR2B subunit in Western blots of most brain regions. Immunolabeling blocked by pre-adsorption of antibody with the C-terminal fusion protein of the NR2B subunit that was used to generate the antibody. No reactivity towards the NR2A subunit of the NMDAR. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blots (WB). Immunolabeling has been demonstrated in human, mouse and rat tissues. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB, 1:1000-1:2000 for IHC, and in IP, 3 μg/200 μg lysate will quantitatively precipitate all NMDAR 2B.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
ImmunogenNMDA NR2B polyclonal antibody was raised against a C-terminal fusion protein of the NR2B subunit of the NMDAR.
TargetGrin2b
Molecular Weight180
PurificationAffinity Purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Grin2b Antibody

NMDA NR2B Subunit Antibody

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqQ00960

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
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IP
Immunoprecipitation
View Protocol

Grin2b Antibody (orb1273486)

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Available Sizes

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10 μg
$ 1,000.00
DispatchUsually dispatched within 5-10 working days
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