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NLK-T286 Antibody (Center)

Catalog Number: orb1929169

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1929169
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB16621
Tested applicationsFC, IF, WB
Predicted ReactivityMouse, Rat
ReactivityHuman
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeIF: 1:10~50, WB: 1:1000, FC: 1:10~50
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW58283 Da
TargetThis NLK-T286 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-300 amino acids from the Central region of human NLK-T286.
UniProt IDQ9UBE8
NCBINP_057315.3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesSerine/threonine-protein kinase NLK, Nemo-like kin
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NoteFor research use only
Expiration Date12 months from date of receipt.
NLK-T286 Antibody (Center)

NLK-T286 western blot analysis in Y79 cell line lysates (35 ug/lane).This demonstrates the NLK antibody detected the NLK protein (arrow).

NLK-T286 Antibody (Center)

Confocal immunofluorescent analysis of NLK-T286 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

NLK-T286 Antibody (Center)

NLK-T286 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.