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Catalog Number | orb1929169 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB16621 |
Tested applications | FC, IF, WB |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 58283 Da |
Target | This NLK-T286 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-300 amino acids from the Central region of human NLK-T286. |
UniProt ID | Q9UBE8 |
NCBI | NP_057315.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Serine/threonine-protein kinase NLK, Nemo-like kin Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
NLK-T286 western blot analysis in Y79 cell line lysates (35 ug/lane).This demonstrates the NLK antibody detected the NLK protein (arrow).
Confocal immunofluorescent analysis of NLK-T286 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
NLK-T286 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.