NLK-T286 Antibody (Center)

• Catalog Number: orb1929169
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This NLK-T286 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-300 amino acids from the Central region of human NLK-T286.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9UBE8
• MW: 58283 Da
• Tested applications: FC, IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB16621
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Serine/threonine-protein kinase NLK, Nemo-like kin
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_057315.3

NLK-T286 western blot analysis in Y79 cell line lysates (35 ug/lane).This demonstrates the NLK antibody detected the NLK protein (arrow).

Confocal immunofluorescent analysis of NLK-T286 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

NLK-T286 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.