NIPA4 Antibody (N-term)

• Catalog Number: orb1939180
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB27750
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 44005 Da
• Target: This NIPA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human NIPA4.
• UniProt ID: Q0D2K0
• NCBI: NP_001165763.1, NP_001092757.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Magnesium transporter NIPA4, Ichthyin, NIPA-like p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-NIPA4 Antibody (N-term) at 1:1000 dilution + human brain lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

NIPA4 Antibody (N-term) western blot analysis in mouse liver tissue lysates (15 ug/lane). This demonstrates the NIPA4 antibody detected NIPA4 protein (arrow).

NIPA4 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NIPA4 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.