Nexturastat A

SKU: orb1307382

Description

Nexturastat A is a potent and highly selective HDAC6 inhibitor with an IC50 of 5 nM, demonstrating over 190-fold selectivity against other HDAC isoforms. This specificity makes it a valuable chemical probe for investigating HDAC6's role in oncology, neurology, and immunology in both cellular and animal models.

Research Area

Epigenetics & Chromatin, Molecular Biology

Key Properties

• CAS Number: 1403783-31-2
• MW: 341.4
• Purity: 98.98% (May vary between batches)
• Formula: C₁₉H₂₃N₃O₃
• SMILES: C(N(C(NC1=CC=CC=C1)=O)CCCC)C2=CC=C(C(NO)=O)C=C2
• Target: HDAC
• Solubility: DMSO:63 mg/mL (184.53 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:2 mg/mL (5.86 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.86 mM)

Bioactivity

• Target IC50:
  HDAC11:5.14 μM|HDAC10:7.57 μM|HDAC3:6.68 μM|HDAC8:0.954 μM|HDAC6:5 nM|HDAC7:4.46 μM|HDAC9:6.72 μM|HDAC5:11.7 μM|HDAC2:6.92 μM|HDAC1:3.02 μM|HDAC4:9.39 μM
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• In Vivo:
  Nexturastat A significantly inhibits the growth of B16 melanoma cells in mice, with a GI50 of 14.3 μM. In these cells, Nexturastat A dose-dependently increases the levels of acetylated α-tubulin without affecting the acetylation of histone H3 associated with abscesses.
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• Cell Research:
  B16 murine melanoma cells are plated at 5000/well in 96 well flat bottom plates. The following day, media is changed to that containing various concentrations of HDACi or matched DMSO vehicle concentrations diluted in complete medium done in triplicate. Cells are incubated for 48 hours at 37°C and 5% CO2. Density of viable, metabolically active cells is quantified using a standard MTS assay as per manufacturer’s instructions. Briefly, 20μL of reagent are added per well and incubated at 37°C for 3 hours. Absorbances at 490 nM are measured spectrophotometrically with background subtraction at 690 nM. All values are then normalized and expressed as a percentage of medium control (100%). (Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nexturastat A, inhibit, Histone deacetylases, Inhibitor, HDAC6, HDAC

Compound Identifiers

PubChem CID

71462653