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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human CD113, Fc Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Human TIGIT, His Tag with a linear range of 0.3-5 ug/mL. |
| Target | Nectin-3 |
| Tag | Fc Tag |
| MW | 64.3 kDa |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Gly 58 - Asp 400 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human Nectin-3 Protein [orb2994964]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 34.96 KDa. Observed: 54 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human CD113/NECTIN3 Protein, C-His [orb2966337]
ELISA, SDS-PAGE, WB
>95% as determined by SDS-PAGE.
40.97 kDa
1 mg, 50 μg, 100 μgRecombinant Human CD113/NECTIN3 Protein, N-His [orb2967258]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
22.84 kDa
1 mg, 50 μg, 100 μgNectin-3 Recombinant Protein [orb1227736]
WB
>95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method.
39.6 kDa
HEK293 cells
0.1 mg

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Immobilized Human CD113, Fc Tag at 10 ug/ml (100 uL/well) can bind Biotinylated Human TIGIT, His Tag with a linear range of 0.3-5 ug/ml.
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Protocol Information
Nectin-3 Recombinant Protein (orb1227735)
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