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Description
Research Area
Cancer Biology, Immunology & Inflammation, Metabolism Research, Neuroscience, Signal Transduction
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Shows adipogenic effects in stimulated differentiating 3T3-L1 cells. |
| Target | NAMPT |
| Tag | His Tag |
| Purity | >90% (SDS-PAGE). Endotoxin level is less than 1EU/ μg purified protein (LAL test; Lonza). |
| Protein Sequence | Intracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag. |
Storage & Handling
−| Storage | Working aliquots are stable for up to 3 months when stored at -20°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Salt-free. Reconstitute in distilled water. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Pre-B Cell Colony Enhancing Factor 1, PBEF1, Nicotinamide Phosphoribosyltransferase
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Rabbit
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50 μl, 100 μl, 200 μl, 30 μlRecombinant Human NAMPT Protein [orb3002311]
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 57 KDa. Observed: 55 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
Nampt Recombinant Protein (orb1230280)
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