N1-Methylpseudouridine

SKU: orb1307015

Description

N1-Methylpseudouridine is a modified nucleoside that augments mRNA translation by boosting ribosome density through both eIF2α-dependent and independent pathways. It is widely used in mRNA vaccine research and therapeutic development to enhance protein expression in both in vitro and in vivo systems.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 13860-38-3
• MW: 258.23
• Purity: >99.99% (May vary between batches)
• Formula: C₁₀H₁₄N₂O₆
• SMILES: Cn1cc([C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)c(=O)[nH]c1=O
• Target: DNA/RNA Synthesis,Others,Nucleoside Antimetabolite/Analog
• Solubility: H2O:40 mg/mL (154.9 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (15.49 mM);DMSO:100 mg/mL (387.25 mM)

Bioactivity

• In Vivo:
  mRNAs containing the N1-Methylpseudouridine modification alone and/or in combination with 5-methylcytidine (m5C) outperformed the current state-of-the-art pseudouridine (Ψ) and/or m5C/Ψ-modified mRNA platform by providing up to ~44-fold (when comparing double modified mRNAs) or ~13-fold (when comparing single modified mRNAs) higher reporter gene expression upon transfection into cell lines or mice, respectively. We show that (m5C/)N1-Methylpseudouridine-modified mRNA resulted in reduced intracellular innate immunogenicity and improved cellular viability compared to (m5C/)Ψ-modified mRNA upon in vitro transfection.
• In Vitro:
  In HEK293T cells, Incorporation of N1-Methylpseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA . Incorporation of N1-Methylpseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation. In addition, polysome formation and growth on the N1-Methylpseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ribosome, protein, polysomes, phosphorylation, 1-Methylpseudouridine, cells, DNA/RNA Synthesis, Nucleoside Antimetabolite/Analog, N1 Methylpseudouridine, N1Methylpseudouridine, N-1-Methylpseudouridine, N1-Methylpseudouridine, inhibit, mRNA, GFP, eIF2α, expression, HEK293T, Inhibitor

Compound Identifiers

PubChem CID

99543