N-Phenylthiourea

SKU: orb1737485

Description

N-Phenylthiourea (Phenylthiocarbamide) is a tyrosinase inhibitor (EC 1.14.18.1) that suppresses melanogenesis and acts as a non-competitive inhibitor of bacterial PvdP tyrosinase. It is widely used in genetic taste perception studies and in biochemical research involving melanin production both in vitro and in microbial systems.

Research Area

Metabolism Research, Protein Biochemistry

Key Properties

• CAS Number: 103-85-5
• MW: 152.22
• Purity: 99.71% (May vary between batches)
• Formula: C₇H₈N₂S
• SMILES: S=C(N)NC=1C=CC=CC1
• Target: Others,Endogenous Metabolite,Tyrosinase
• Solubility: DMSO:80 mg/mL (525.56 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (21.68 mM)

Bioactivity

• In Vivo:
  In zebrafish embryos, co-treatment with N-Phenylthiourea (100 μM) and HgCl₂ significantly reduced mercury toxicity, with both mortality and hepatic Hg levels decreasing by 60-fold. In contrast, co-treatment with CH₃HgCl increased toxicity, leading to higher mortality and a 2-fold increase in hepatic Hg accumulation. These results indicate that N-Phenylthiourea has opposite modulatory effects on the toxicity of inorganic and organic mercury.
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• In Vitro:
  N-Phenylthiourea acts as a reversible competitive inhibitor of tyrosinase in DOPA oxidation assays (Ki = 0.21 μM), significantly reducing DOPA-chrome formation.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Phenylthiourea, Phenylthiocarbamide

Compound Identifiers

PubChem CID

676454