N-Aminofluorescein

SKU: orb2900267

Description

N-Aminofluorescein is a fluorescein hydrazide with spiro form, a highly selective and sensitive fluorescence probe for Cu2+. N-Aminofluorescein has no selective fluorescence response to other common metal ions, can be used for direct detection of Cu2+ in biological systems with λex/em=495/516 nm.

Key Properties

• CAS Number: 98907-26-7
• MW: 346.34
• Purity: >98% (HPLC)
• Formula: C₂₀H₁₄N₂O₄
• SMILES: O=C1C=2C=CC=CC2C3(C4=CC=C(O)C=C4OC5=CC(O)=CC=C53)N1N
• Solubility: In Vitro: DMSO : 125 mg/mL (360.92 mM; Ultrasonic )

Bioactivity

• In Vitro:
  Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). N-Aminofluorescein consists of a fluorescein moiety and a hydrazide group to recognize and bind Cu2+, can promote the hydrolysis of amide. N-Aminofluorescein (FG) shows selectivity on Cu2+ and shows the absorption and emission bands at 632 nm and 515 nm in 70% aqueous HEPES buffered solution (pH 7.4) containing Cu2+. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). General procedure for Cu2+ determination: 1.Prepare the 1.0 mM stock solution of spiro form fluorescein hydrazide in ethanol; Conduct the fluorescence-on reaction in 0.01 M Tris-HCl buffer (pH 7.2), with 10 μM N-Aminofluorescein; 3.Add an appropriate volume of sample solution with a final Cu2+ concentration of not more than 10 μM, and adjust the final volume 10 mL with 0.01 M Tris-HCl buffer (pH 7.2); 4.After 2 h, transfer a 3-mL portion of the solution to a 1-cm quartz cell, and measure the fluorescence intensity/spectrum at room temperature with λex/em = 495/516 nm and both excitation and emission slit widths of 5 nm; 5.In the meantime, prepare a blank solution containing no Cu2+ and measure with the same conditions for comparison.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only