Myelin Basic Protein antibody (MSVA-390R)

• Catalog Number: orb2276388
• Category: Antibodies
• Description: Myelin Basic Protein antibody validated for immunohistochemistry on 76 different Normal Tissues
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant fragment (around aa 150-250) of human MBP (exact sequence is proprietary)
• UniProt ID: P02686
• Tested applications: IHC
• Dilution range: 1:100-1:200
• Application notes: Positive Control: Brain: A fibrillar staining pattern should be seen. Negative Control: Colon: MBP staining should be completely absent. Cellular Localization: Cell surface and cytoplasm. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Clone Number: Biorbyt-390R
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: GDB; Golli MBP; myelin basic protein; Hemopoietic
• Note: For research use only

In the cerebellum a fibrillar MBP immunostaining occurs along intracerebral axons.

In the cerebrum a fibrillar MBP immunostaining occurs along intracerebral axons. Neurons are not stained.

Complete absence of MBP immunostaining in epithelial and stromal cells of the appendix mucosa.