Mouse TIGIT Protein, His Tag

Catalog Number: orb348797

Catalog Number	orb348797
Category	Proteins
Description	Mouse TIGIT, His Tag is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Thr 143 (Accession # NP_001139797.1).
Reactivity	Mouse
Tag	C-10×His
Form/Appearance	Powder
Purity	95%
Conjugation	Unconjugated
MW	14.8 kDa
Target	TIGIT
UniProt ID	A0A0B4J1G6
Protein Sequence	NP_001139797.1
Expression System	HEK293
Biological Origin	Mouse
Expression Region	Gly 26 - Thr 143
Endotoxins	1.0 EU per μg
NCBI	NP_001139797.1
Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Buffer/Preservatives	PBS, pH 7.4
Note	For research use only
Application notes	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.8 kDa. The protein migrates as 18-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expiration Date	6 months from date of receipt.

SDS-PAGE analysis of Mouse TIGIT protein

Purity:	> 95% by SDS-PAGE.
Source:	Recombinant Mouse TIGIT Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Gly26-Phe135) of mouse TIGIT (Accession #NP_001139797) fused with a 6xHis Tag at the C- terminus.

Sizes Available: 50 μg

Purity:	98.00%
MW:	16.01 kDa (predicted); 26.6 kDa (reducing conditions)

Purity:	SDS-PAGE: 94%; SEC-HPLC: 90.7%
MW:	14.2 kDa (predicted); 20-25 kDa (reducing condition, due to glycosylation)

Purity:	> 90%, determined by SDS-PAGE
Source:	This protein contains the extracellular domain of mouse TIGIT (NP_001139797.1) (Met 1-Gly 141) was expressed from HEK293 Cells.