Mouse Sirt1 Antibody (C-term)

• Catalog Number: orb1926867
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Mouse Sirt1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 566-601 amino acids from the C-terminal region of human Mouse Sirt1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q923E4
• MW: 80372 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:25, WB: 1:2000, WB: 1:1000, IHC-P: 1:25
• Clone Number: RB50965
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: NAD-dependent protein deacetylase sirtuin-1, 351-,
• Note: For research use only

Western blot analysis of lysate from mouse F9 cell line, using Mouse Sirt1 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Anti-Sirt1 Antibody (C-term) at 1:2000 dilution + MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded M. brain section using Mouse Sirt1 Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of A549 cells stained with Mouse Sirt1 Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).