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Catalog Number | orb257730 |
---|---|
Category | Proteins |
Description | Mouse OX40, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 20 - Pro 211 (Accession # P47741-1). |
Reactivity | Mouse |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 23.2 kDa |
Target | OX40 |
UniProt ID | P47741 |
Protein Sequence | NP_035789.1 |
Expression System | HEK293 |
Biological Origin | Mouse |
Expression Region | Val 20 - Pro 211 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_035789.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.2 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse OX40 protein
Line graph illustrates about the sensitivity range of Mouse OX40 protein using different concentrations
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 44.0 kDa after removal of the signal peptide. | |
Mammalian |
98.00% | |
24.2 kDa (predicted). Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result. |
98.00% | |
24.2 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
98.00% | |
40 KDa (reducing condition) |
Greater than 90.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |