Mouse Interferon Gamma (IFN-γ) ELISA Kit

SKU: orb1806800

Description

Mouse Interferon Gamma (IFN-γ) ELISA Kit

Images & Validation

• Application Notes:
  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-γ. You can calculate the concentration of Mouse IFN-γ in the samples by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, Tissue homogenate and Other biological samples
• Assay Type: Sandwich
• Assay Time: 3.5H
• Range: 15.63-1000pg/mL
• Sensitivity: 9.38 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IFNG, IFG, IFI, Type II Interferon

Quick Database Links

Gene Symbol

IFN-γ

UniProt

P01580

References

Zichao Yang 1, Pan Yang 2 3, Jianwei Xu 1, Xixiang Yang 1, Jiayi Zhou 1, Haiqi He 1, Ling Li 4, Yichang Ren 1, Meirong Chen 2 3, Yibei Xiao 2 3, Jianjun Chen 1 Discovery and Crystallography Study of Novel Resorcinol Dibenzyl Ether-Based PD-1/PD-L1 Inhibitors with Improved Drug-like and Pharmacokinetic Properties for Cancer Treatment J Med Chem, (2025)

PubMed: 40518797

Applications

ELISA

Reactivity

Mouse