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Mouse IgG2b Lambda Isotype control Antibody

Catalog Number: orb343766

DispatchUsually dispatched within 5-10 working days
$ 400.00
Catalog Numberorb343766
CategoryProteins
DescriptionMouse IgG2b Lambda Isotype control Antibody
Tested applicationsFC, SDS-PAGE
IsotypeIgG2b
Concentration1.01 mg/mL
Dilution rangeFC: 1:1000-1:5000
Form/AppearanceLiquid (sterile filtered)
PurityMouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.
ConjugationUnconjugated
SourceMouse
Biological OriginMouse
StorageStore vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesMouse subclass antibodies, IgG2 subclass, Mouse Ig
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NoteFor research use only
Application notesMouse IgG2b lambda control has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
Expiration Date12 months from date of receipt.
Mouse IgG2b Lambda Isotype control Antibody

SDS-PAGE Results of Mouse IgG2b Lambda Isotype Control. Lane 1: Mouse IgG2b Lambda Isotype Control (Reduced) [5.0 µg]. Lane 2: Opal Prestained Molecular Weight Markers. Lane 3: Mouse IgG2b Lambda Isotype Control (Non-Reduced) [5.0 µg]. 4-20% Gel, Coomassie Stained.