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Catalog Number | orb343766 |
---|---|
Category | Proteins |
Description | Mouse IgG2b Lambda Isotype control Antibody |
Tested applications | FC, SDS-PAGE |
Isotype | IgG2b |
Concentration | 1.01 mg/mL |
Dilution range | FC: 1:1000-1:5000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE. |
Conjugation | Unconjugated |
Source | Mouse |
Biological Origin | Mouse |
Storage | Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Mouse subclass antibodies, IgG2 subclass, Mouse Ig Read more... |
Note | For research use only |
Application notes | Mouse IgG2b lambda control has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE Results of Mouse IgG2b Lambda Isotype Control. Lane 1: Mouse IgG2b Lambda Isotype Control (Reduced) [5.0 µg]. Lane 2: Opal Prestained Molecular Weight Markers. Lane 3: Mouse IgG2b Lambda Isotype Control (Non-Reduced) [5.0 µg]. 4-20% Gel, Coomassie Stained.