Mouse IgG2a isotype Control Antibody

• Catalog Number: orb343777
• Category: Proteins
• Description: Mouse IgG2a isotype Control Antibody
• Tested applications: FC, SDS-PAGE
• Isotype: IgG2a
• Concentration: 1.0 mg/mL
• Dilution range: FC: 1:1000-1:5000
• Form/Appearance: Liquid
• Purity: Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Confirmed by SDS-PAGE.
• Conjugation: Unconjugated
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: MOUSE IgG2a isotype Control
• Note: For research use only
• Application notes: Mouse IgG2a isotype control has been tested by SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
• Expiration Date: 12 months from date of receipt.

(E) Cell surface-bound gp130 was down-regulated in OVCAR-8 by SC144 at the indicated concentration and time. After treatment, cells were collected and stained with anti-gp130 (B-R3) antibody and analyzed by FACS. Upper panel, one of three representative experiments. Lower panels, statistical analyses of data from the three experimental repeats. Error bars = SEM. * P < 0.05, ** P < 0.01, paired t test. Mouse IgG2a (p/n orb343777).

SDS-Page Results of Mouse IgG2a Isotype Control. Lane 1: Mouse IgG2a Isotype Control Reduced [1.0 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Mouse IgG2a Isotype Control Non-Reduced [1.0 µg]. 4-20% gel. Coomassie stained.