Mouse IgG2a isotype Control Antibody

SKU: orb343777

Description

Mouse IgG2a isotype Control Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: FC, SDS-PAGE
• Dilution Range: FC: 1:1000-1:5000
• Application Notes:
  Mouse IgG2a isotype control has been tested by SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Key Properties

• Isotype: IgG2a
• Clone No.: M2A
• Purity: Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Confirmed by SDS-PAGE.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MOUSE IgG2a isotype Control

(E) Cell surface-bound gp130 was down-regulated in OVCAR-8 by SC144 at the indicated concentration and time. After treatment, cells were collected and stained with anti-gp130 (B-R3) antibody and analyzed by FACS. Upper panel, one of three representative experiments. Lower panels, statistical analyses of data from the three experimental repeats. Error bars = SEM. * P < 0.05, ** P < 0.01, paired t test. Mouse IgG2a (p/n orb343777).

SDS-Page Results of Mouse IgG2a Isotype Control. Lane 1: Mouse IgG2a Isotype Control Reduced [1.0 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Mouse IgG2a Isotype Control Non-Reduced [1.0 µg]. 4-20% gel. Coomassie stained.