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Catalog Number | orb347514 |
---|---|
Category | Antibodies |
Description | Mouse IgG (gamma 1, 2a, 2b and 3 chain) antibody (Texas Red) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, FLISA, IF, IHC, WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins |
Concentration | 1.0 mg/mL |
Dilution range | FLISA: 1:20,000 - 1:100,000, FC: 1:1,000 - 1:5,000, IHC: 1:1,000 - 1:5,000, IF: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. |
Conjugation | Texas Red |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain Read more... |
Note | For research use only |
Application notes | Anti-Mouse IgG subclass pan reactive Texas Red Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti-mouse IgG antibody Texas Red is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
Expiration Date | 12 months from date of receipt. |
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